2FRK
Nitrosyl Horse Heart Myoglobin, Nitric Oxide Gas Method
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9611 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.516, 28.605, 63.294 |
| Unit cell angles | 90.00, 105.65, 90.00 |
Refinement procedure
| Resolution | 9.890 - 1.300 |
| R-factor | 0.17028 |
| Rwork | 0.168 |
| R-free | 0.20477 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.138 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9.890 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.055 | |
| Number of reflections | 30078 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 98.7 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | Ammonium Sulfate, Tris-HCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
