2FRI
Horse Heart Myoglobin, Nitrite Adduct, Co-crystallized
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.223, 28.592, 62.841 |
| Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.600 |
| R-factor | 0.14905 |
| Rwork | 0.146 |
| R-free | 0.20644 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1npf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.135 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.840 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.047 | 0.247 |
| Number of reflections | 16051 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 98.5 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | Ammonium Sulfate, Tris-HCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
