2FRH
Crystal Structure of Sara, A Transcription Regulator From Staphylococcus Aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2001-09-01 |
Detector | PRINCETON 2K |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 65.338, 65.338, 237.289 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.500 - 2.500 |
R-factor | 0.266 |
Rwork | 0.266 |
R-free | 0.30200 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.072 |
Number of reflections | 10107 |
<I/σ(I)> | 33 |
Completeness [%] | 89.7 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | PEG 4000, PH 6.5, VAPOR DIFFUSION, HANGING DROP AT 277K, pH 6.50 |