2FR3

Crystal Structure of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinoic Acid at 1.48 Angstroms Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2005-07-23
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	44.287, 46.464, 77.261
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.810 - 1.480
R-factor	0.131
Rwork	0.126
R-free	0.17400
Structure solution method	Rigid Body Refinement
RMSD bond length	0.019
RMSD bond angle	1.815
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.810	1.530
High resolution limit [Å]	1.480	1.480
Rmerge	0.044	0.139
Number of reflections	26090	2590
<I/σ(I)>	28.19	5.08
Completeness [%]	95.5	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	30% (w/v) PEG 4000, 0.1 M sodium citrate/citric acid pH 5.4, and 0.2 ammonium acetate (crystallization performed in dark room and under red light), VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	30% (w/v) PEG 4000, 0.1 M sodium citrate/citric acid pH 5.4, and 0.2 ammonium acetate (crystallization performed in dark room and under red light), VAPOR DIFFUSION, HANGING DROP, temperature 298K