2FQM

Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-08
Detector	MARRESEARCH
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	74.380, 74.380, 157.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.227
Rwork	0.225
R-free	0.29000
Structure solution method	SAD
RMSD bond length	0.026
RMSD bond angle	2.263
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300	2.300
Rmerge	0.065
Number of reflections	20381
<I/σ(I)>	22.62
Completeness [%]	99.8	99.8
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.32	298	2.48 M ammonium sulfate, 7% ethylene glycol, 0.05% n-octyl-b-D-glucopyranoside, pH 4.32, VAPOR DIFFUSION, HANGING DROP, temperature 298K