2FQL
Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-26 |
| Detector | MARRESEARCH |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 121.220, 121.220, 121.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.740 - 3.010 |
| R-factor | 0.286 |
| Rwork | 0.286 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1EKG AND 1EW4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.900 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.190 |
| High resolution limit [Å] | 3.010 | 3.010 |
| Number of reflections | 6059 | |
| Completeness [%] | 98.3 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 298 | 2 M AmSO4, 0.1 M BIS-TRIS, 4% GAMMA-Butyrolactone, pH 5.5, VAPOR DIFFUSION, temperature 298K, pH 5.50 |
