2FQ3
Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2003-08-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1, 0.9788, 0.9791, 0.9562 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.226, 44.309, 48.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.400 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.21300 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.040 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 19520 | |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris HCl (pH 8.5), 10% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
