2FPW
Crystal Structure of the N-terminal Domain of E.coli HisB- Phosphoaspartate intermediate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.983, 132.408, 105.685 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.17925 |
| Rwork | 0.178 |
| R-free | 0.21104 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2fpr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.281 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 36862 | |
| <I/σ(I)> | 17.1 | 7.1 |
| Completeness [%] | 96.8 | 92 |
| Redundancy | 5.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 293 | 30% (w/v) PEG monomethylether 550, 0.05M CaCl2, 0.1M Bis-Tris., pH 6.5, EVAPORATION, temperature 293K |
