2FPP
Crystal structure of pig GTP-specific succinyl-CoA synthetase from polyethylene glycol with chloride ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.410, 123.410, 84.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.350 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.114 | 0.374 |
| Number of reflections | 27045 | 2359 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 87.9 | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 10% v/v isopropanol, 100 mM ammonium sulfate, 20% w/v polyethylene glycol 400, 100 mM HEPES (pH 7.5 with HCl), VAPOR DIFFUSION, HANGING DROP, temperature 294K |
