2FPP
Crystal structure of pig GTP-specific succinyl-CoA synthetase from polyethylene glycol with chloride ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-01-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 |
Unit cell lengths | 123.410, 123.410, 84.860 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 100.000 - 2.350 |
R-factor | 0.195 |
Rwork | 0.192 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.114 | 0.374 |
Number of reflections | 27045 | 2359 |
<I/σ(I)> | 9.2 | |
Completeness [%] | 87.9 | 0.773 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 10% v/v isopropanol, 100 mM ammonium sulfate, 20% w/v polyethylene glycol 400, 100 mM HEPES (pH 7.5 with HCl), VAPOR DIFFUSION, HANGING DROP, temperature 294K |