2FPN
The crystal structure of the ywmB protein from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.790, 50.856, 81.259 |
| Unit cell angles | 90.00, 96.49, 90.00 |
Refinement procedure
| Resolution | 80.850 - 2.490 |
| R-factor | 0.24446 |
| Rwork | 0.242 |
| R-free | 0.28134 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.386 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.000 | 2.550 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.071 | 0.438 |
| Number of reflections | 11820 | |
| <I/σ(I)> | 34 | 2.37 |
| Completeness [%] | 96.4 | 74.46 |
| Redundancy | 4.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% PEG4000, 45% tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
