2FPH
Cell division protein ylmH from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-17 |
Detector | MACSCIENCE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.180, 46.267, 50.261 |
Unit cell angles | 90.00, 99.70, 90.00 |
Refinement procedure
Resolution | 49.390 - 1.700 |
R-factor | 0.243 |
Rwork | 0.240 |
R-free | 0.29900 |
Structure solution method | SIRAS |
RMSD bond length | 0.021 |
RMSD bond angle | 2.155 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.068 | 0.217 |
Number of reflections | 17137 | |
<I/σ(I)> | 5.2 | 2.8 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.1 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 293 | 10% PEG6000, 1.0M LiCl, 100mM citrate buffer, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 5.00 |