2FPG
Crystal structure of pig GTP-specific succinyl-CoA synthetase in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.11589 |
| Spacegroup name | P 65 |
| Unit cell lengths | 133.780, 133.780, 73.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 115.858 - 2.960 |
| R-factor | 0.205 |
| Rwork | 0.201 |
| R-free | 0.27500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 115.858 | 116.250 | 3.120 |
| High resolution limit [Å] | 2.960 | 9.360 | 2.960 |
| Rmerge | 0.126 | 0.053 | 0.532 |
| Total number of observations | 3155 | 12795 | |
| Number of reflections | 15727 | 528 | 2281 |
| <I/σ(I)> | 5.5 | 11.1 | 1.4 |
| Completeness [%] | 100.0 | ||
| Redundancy | 6.3 | 6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 5 mM GDP, 10 mM MgCl2, 20% w/v polyethylene glycol 3350, 150 mM KF, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
