2FPG

Crystal structure of pig GTP-specific succinyl-CoA synthetase in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.11589
Spacegroup name	P 65
Unit cell lengths	133.780, 133.780, 73.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	115.858 - 2.960
R-factor	0.205
Rwork	0.201
R-free	0.27500
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	115.858	116.250	3.120
High resolution limit [Å]	2.960	9.360	2.960
Rmerge	0.126	0.053	0.532
Total number of observations		3155	12795
Number of reflections	15727	528	2281
<I/σ(I)>	5.5	11.1	1.4
Completeness [%]	100.0
Redundancy	6.3	6	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	5 mM GDP, 10 mM MgCl2, 20% w/v polyethylene glycol 3350, 150 mM KF, VAPOR DIFFUSION, HANGING DROP, temperature 294K