2FPD
Sad structure determination: crystal structure of the intrinsic dimerization sh3 domain of the ib1 scaffold protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 114.486, 114.486, 48.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.640 - 2.050 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.096 | 0.340 |
| Number of reflections | 37925 | |
| <I/σ(I)> | 7.7 | 2.28 |
| Completeness [%] | 98.3 | 97.4 |
| Redundancy | 8.02 | 8.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | AMMONIUM SULFATE, BICINE, pH 9.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
