2FOT

Crystal structure of the complex between calmodulin and alphaII-spectrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2005-11-24
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	44.288, 57.737, 69.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.480 - 2.450
R-factor	0.245
Rwork	0.245
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cdm
RMSD bond length	0.008
RMSD bond angle	1.230
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.600
High resolution limit [Å]	2.450	2.450
Rmerge	0.245	0.467
Number of reflections	7933
<I/σ(I)>	4.55	2.2
Completeness [%]	89.8	87.1
Redundancy	5.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	50mM Tris,pH 8.00, 30% PEG 8000, 0.1M ammonium-sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	8	298	50mM Tris,pH 8.00, 30% PEG 8000, 0.1M ammonium-sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K