2FON

X-ray crystal structure of LeACX1, an acyl-CoA oxidase from Lycopersicon esculentum (tomato)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-28
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	120.200, 240.330, 89.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.740
Rwork	0.212
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.863
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.770
High resolution limit [Å]	2.700	6.490	2.700
Rmerge	0.092	0.078	0.374
Number of reflections	64138	5105	4704
<I/σ(I)>	12.2
Completeness [%]	95.4
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	296	10% 2-methyl-2,4-pentanediol, 7% PEG 6000, 100 mM Tris, 1 mM acetyl-CoA, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	8	296	10% 2-methyl-2,4-pentanediol, 7% PEG 6000, 100 mM Tris, 1 mM acetyl-CoA, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K