2FOK

STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	130
Detector technology	IMAGE PLATE
Collection date	1997-01-20
Detector	FUJI
Spacegroup name	P 21 21 21
Unit cell lengths	54.010, 137.170, 188.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.211
Rwork	0.211
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fok
RMSD bond length	0.007
RMSD bond angle	25.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.082	0.364
Number of reflections	61150
<I/σ(I)>	21.3	4.4
Completeness [%]	95.9	81.9
Redundancy	13.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5		PROTEIN CRYSTALLIZED IN 22% PEG 8000, 0.4 M AMMONIUM SULFATE, 0.1 M SODIUM CACODYLATE, PH 6.5.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.12 (mM)
10	1	drop	glycerol	5 (%)
11	1	reservoir	PEG8000	22 (%)
12	1	reservoir	ammonium sulfate	0.4 (M)
13	1	reservoir	sodium cacodylate	0.1 (M)	pH6.5
2	1	drop	PEG8000	11 (%)
3	1	drop	ammonium sulfate	0.2 (M)
4	1	drop	sodium cacodylate	50 (mM)	pH6.5
5	1	drop		0.2 (M)
6	1	drop	potassium phosphate	10 (mM)
7	1	drop	dithiothreitol	0.5 (mM)
8	1	drop	EDTA	0.5 (mM)
9	1	drop	EGTA	0.5 (mM)