2FOG
Structure of porcine pancreatic elastase in 40% trifluoroethanol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-6 |
Temperature [K] | 277 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS-NICOLET X100 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.600, 58.070, 75.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 - 1.900 |
Rwork | 0.172 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1elc |
RMSD bond length | 0.005 |
RMSD bond angle | 1.225 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 500.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 14069 |
Completeness [%] | 40.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 15 mg/ml elastase, 25 mM Sodium Acetate, 135 mM Sodium sulfate, 10 ul drop (5x5). See also: Sawyer et al., J.Mol.Biol. 118, 137-208 (1978), pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |