2FO7
Crystal structure of an 8 repeat consensus TPR superhelix (trigonal crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.60 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.550, 68.550, 67.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.266 |
| Rwork | 0.266 |
| R-free | 0.30400 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.02) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.041 | 0.325 |
| Number of reflections | 15329 | 968 |
| <I/σ(I)> | 17.2 | 2.3 |
| Completeness [%] | 95.0 | 61.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 25% MPD, 10 mM CDCL2, 100 mM NA-ACETATE, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
