2FO3
Plasmodium vivax ubiquitin conjugating enzyme E2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 58.004, 58.004, 117.114 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.860 |
| R-factor | 0.20863 |
| Rwork | 0.204 |
| R-free | 0.26251 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2u |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.454 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (V. 1.3.1) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.064 | 0.978 |
| Number of reflections | 10446 | |
| <I/σ(I)> | 10.1 | 2.38 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 14.4 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 22% PEG3350, 0.19M Ca(AC)2, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
