2FNP
Crystal structure of SarA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 52.074 |
| Detector technology | CCD |
| Collection date | 2005-03-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.074, 64.758, 55.564 |
| Unit cell angles | 90.00, 117.73, 90.00 |
Refinement procedure
| Resolution | 39.170 - 2.600 |
| R-factor | 0.285 |
| Rwork | 0.285 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.068 | |
| Number of reflections | 9772 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 96.0 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | 25% PEG 8000, 0.2M calcium chloride, 0.1M cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 277K |
