2FNI

PseC aminotransferase involved in pseudoaminic acid biosynthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-11-28
Detector	RIGAKU RAXIS HTC
Spacegroup name	P 21 21 2
Unit cell lengths	87.400, 153.300, 70.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.000
R-factor	0.19943
Rwork	0.196
R-free	0.25794
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fn6
RMSD bond length	0.008
RMSD bond angle	1.062
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.098
Number of reflections	19466
<I/σ(I)>	7.1
Completeness [%]	95.1
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	protein (5 mg/ml) in buffer (20 mM citrate, pH 5.4, 0.1 M NaCl, 5% (v/v) glycerol, and 10 mM DTT) was incubated with 0.5 mM PLP. Crystals of the PseC-PLP complex were grown by mixing 1.5 microL protein and 1.5 microL reservoir solution (21% PEG 3350, 0.21 M sodium formate) by vapor diffusion against reservoir solution, VAPOR DIFFUSION, HANGING DROP, temperature 293K