2FNC
Thermotoga maritima maltotriose binding protein bound with maltotriose.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.836, 56.183, 88.794 |
Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.195 |
Rwork | 0.194 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mbp |
RMSD bond length | 0.013 |
RMSD bond angle | 1.321 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.690 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 43576 | |
<I/σ(I)> | 1.8 | |
Completeness [%] | 98.7 | 99.8 |
Redundancy | 2.5 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.2M Na Acetate, 0.1M Na Cacodylate pH 6.5, 30% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 290K |