2FNC
Thermotoga maritima maltotriose binding protein bound with maltotriose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.836, 56.183, 88.794 |
| Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mbp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.321 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 43576 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 98.7 | 99.8 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.2M Na Acetate, 0.1M Na Cacodylate pH 6.5, 30% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
