2FN6
Helicobacter pylori PseC, aminotransferase involved in the biosynthesis of pseudoaminic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 87.691, 155.424, 71.482 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 77.620 - 2.483 |
R-factor | 0.21043 |
Rwork | 0.207 |
R-free | 0.26931 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mdo |
RMSD bond length | 0.010 |
RMSD bond angle | 1.264 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 77.620 |
High resolution limit [Å] | 2.483 |
Rmerge | 0.075 |
Number of reflections | 35038 |
<I/σ(I)> | 40 |
Completeness [%] | 98.9 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | mixing 1.5 microL of protein in buffer with 1.5 microL of reservoir solution containing 0.2 M ammonium acetate, 0.1 M trisodium citrate pH 5.6, 30% PEG 4000., VAPOR DIFFUSION, HANGING DROP, temperature 293K |