2FN6
Helicobacter pylori PseC, aminotransferase involved in the biosynthesis of pseudoaminic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.691, 155.424, 71.482 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.620 - 2.483 |
| R-factor | 0.21043 |
| Rwork | 0.207 |
| R-free | 0.26931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mdo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.264 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 77.620 |
| High resolution limit [Å] | 2.483 |
| Rmerge | 0.075 |
| Number of reflections | 35038 |
| <I/σ(I)> | 40 |
| Completeness [%] | 98.9 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | mixing 1.5 microL of protein in buffer with 1.5 microL of reservoir solution containing 0.2 M ammonium acetate, 0.1 M trisodium citrate pH 5.6, 30% PEG 4000., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
