2FMT
METHIONYL-TRNAFMET FORMYLTRANSFERASE COMPLEXED WITH FORMYL-METHIONYL-TRNAFMET
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 201.710, 68.060, 86.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 2.800 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.29200 |
| Structure solution method | SIR |
| Starting model (for MR) | 1fmt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.720 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | SHARP |
| Refinement software | CNS (0.3C) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.051 * | 0.350 * |
| Number of reflections | 29912 | |
| <I/σ(I)> | 10.9 | 2.2 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.6 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | mPEG5000 | 23 (%) | |
| 2 | 1 | 1 | ammonium sulfate | 0.2 (M) | |
| 3 | 1 | 1 | ethylene glycol | 8 (%) | |
| 4 | 1 | 1 | protein | 0.060 (mM) | |
| 5 | 1 | 1 | formylase | 0.066 (mM) | |
| 6 | 1 | 1 | 10 (mM) | ||
| 7 | 1 | 1 | 50 (mM) | ||
| 8 | 1 | 1 | MES-Na | 50 (mM) |
