2FMJ
220-loop mutant of streptomyces griseus trypsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-11-27 |
Detector | MARRESEARCH |
Spacegroup name | P 65 |
Unit cell lengths | 58.319, 58.319, 100.337 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 16.580 - 1.650 |
Rwork | 0.157 |
R-free | 0.18900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | XTALVIEW |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 1.650 |
Number of reflections | 22158 |
Completeness [%] | 95.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 298 | 1.8 M ammonium sulphate 10 mM calcium acetate, pH 6.1 200 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |