2FMI
Crystal structure of CheY in complex with CheZ 200-214 solved from a F432 crystal grown in Tris (pH 8.4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 198.280, 198.280, 198.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.840 - 2.302 |
R-factor | 0.19551 |
Rwork | 0.193 |
R-free | 0.21948 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fqw |
RMSD bond length | 0.013 |
RMSD bond angle | 1.217 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 17416 | |
<I/σ(I)> | 22.8 | 8 |
Completeness [%] | 99.7 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 298 | 2M ammonium sulfate, 0.2M lithium sulfate, 0.1M Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |