2FMB
EIAV PROTEASE COMPLEXED WITH AN INHIBITOR LP-130
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09-18 |
Detector | MACSCIENCE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 42.650, 45.360, 56.760 |
Unit cell angles | 90.00, 110.36, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.800 |
R-factor | 0.143 * |
Rwork | 0.143 |
Structure solution method | RIGID BODY REFINEMENT |
Starting model (for MR) | 1fmb |
RMSD bond length | 0.013 |
RMSD bond angle | 0.045 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | PROFFT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.049 | 0.300 |
Number of reflections | 8666 | |
Completeness [%] | 90.9 | 91.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | DMSO | ||
3 | 1 | reservoir | PEG4000 | 20 (%) | |
4 | 1 | reservoir | sodium acetate | 25 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 0.45 (M) | |
6 | 1 | reservoir | DMSO | 6 (%) | |
7 | 1 | reservoir | sodium azide | 0.1 (%) |