2FM9
Structure of Salmonella SipA residues 48-264
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 71.080, 71.080, 95.439 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.784 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.031 | 0.357 |
| Number of reflections | 19209 | |
| Completeness [%] | 99.0 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | hanging drops formed from mixing a 1:1 volume ratio of 20mg/ml protein with an equilibration buffer consisting of 20% PEG6000, 20% glycerol, Na-citrate pH 5.6 and 0.01M adenosine-5 -triphosphate disodium salt (ATP) as an additive., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
