2FLM
Human transthyretin (TTR) complexed with bivalant amyloid inhibitor (6 carbon linker)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-09-11 |
| Detector | MAC Science DIP-2000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 42.776, 84.605, 65.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.190 - 1.650 |
| R-factor | 0.20167 |
| Rwork | 0.198 |
| R-free | 0.23396 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bmz |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.297 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.190 | 1.688 |
| High resolution limit [Å] | 1.650 | 1.645 |
| Number of reflections | 29034 | |
| Completeness [%] | 98.3 | 93.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
