2FLK

Crystal structure of CheY in complex with CheZ(200-214) solved from a F432 crystal grown in CAPS (pH 10.5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793
Spacegroup name	F 4 3 2
Unit cell lengths	198.750, 198.750, 198.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.100
R-factor	0.19765
Rwork	0.197
R-free	0.20736
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fqw
RMSD bond length	0.011
RMSD bond angle	1.184
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	20143
<I/σ(I)>	21.3	6.3
Completeness [%]	99.9	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	298	2M ammonium sulfate, 0.2M lithium sulfate, 0.1M CAPS, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K