2FLH
Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-08-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.844, 1.2547, 1.2580, 1.2703 |
Spacegroup name | P 64 |
Unit cell lengths | 113.790, 113.790, 86.980 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.200 |
R-factor | 0.157 |
R-free | 0.19000 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 2.229 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.070 | 0.639 |
Number of reflections | 189769 | |
<I/σ(I)> | 19.7 | 2.6 |
Completeness [%] | 95.7 | 93.2 |
Redundancy | 6.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 292 | sodium citrate, HEPES, zeatin, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K, pH 7.50 |