2FLH
Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.844, 1.2547, 1.2580, 1.2703 |
| Spacegroup name | P 64 |
| Unit cell lengths | 113.790, 113.790, 86.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.200 |
| R-factor | 0.157 |
| R-free | 0.19000 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.229 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.070 | 0.639 |
| Number of reflections | 189769 | |
| <I/σ(I)> | 19.7 | 2.6 |
| Completeness [%] | 95.7 | 93.2 |
| Redundancy | 6.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 292 | sodium citrate, HEPES, zeatin, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K, pH 7.50 |
