2FLF
Crystal structure of l-fuculose-1-phosphate aldolase from Thermus Thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.405, 101.403, 173.223 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.700 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fk5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.074 | 0.564 |
| Number of reflections | 46644 | |
| Completeness [%] | 99.5 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 28% PEG 4000, 0.1M CITRATE-NaOH, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
