2FLC
Post-Reactive Complex of Restriction Endonuclease HinP1I with Nicked Cognate DNA and Magnesium Ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 100.387, 100.387, 139.369 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.100 - 2.590 |
| R-factor | 0.28 |
| Rwork | 0.275 |
| R-free | 0.32400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ynm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 2.650 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.070 | |
| Number of reflections | 13460 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 99.2 | 95.7 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 289 | 20% PEG1000, 400mM NaCl, 100mM Bis-Tris propane, and 5% ethylene glycol (5mM Magnesium Chloride was soaked into the crystal), pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG1000 | ||
| 2 | 1 | 1 | Bis-Tris propane | ||
| 3 | 1 | 1 | ethylene glycol | ||
| 4 | 1 | 1 | NaCl | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG1000 | ||
| 7 | 1 | 2 | NaCl | ||
| 8 | 1 | 2 | H2O |
