2FL7
S. cerevisiae Sir3 BAH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-20 |
| Detector | BRUKER PROTEUM R |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.633, 44.145, 53.723 |
| Unit cell angles | 90.00, 96.94, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.23242 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.097 |
| Data reduction software | PROTEUM PLUS (2 (BRUKER)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.300 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 18434 | |
| Completeness [%] | 97.0 | 70.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M HEPES, pH 6.5, 200 mM sodium chloride, 10% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
