2FL5
Cofactor-containing antibodies: Crystal structure of the original yellow antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.592, 111.105, 188.454 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8fab |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.099 | 0.812 |
| Number of reflections | 42610 | |
| <I/σ(I)> | 15.5 | 2.1 |
| Completeness [%] | 98.9 | 99.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | PEG 4000, di-ammonium hydrogen citrate, tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 7.50 |
