2FL3
Binary Complex of Restriction Endonuclease HinP1I with Cognate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 100.855, 100.855, 139.406 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.170 - 2.390 |
| R-factor | 0.249 |
| Rwork | 0.246 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ynm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.170 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.079 | |
| Number of reflections | 15524 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 89.0 | 50.2 |
| Redundancy | 20.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 289 | 20% PEG1000, 400mM NaCl, 100mM Bis-Tris propane, and 5% ethylene glycol, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG1000 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | Bis-Tris propane | ||
| 4 | 1 | 1 | ethylene glycol | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | PEG1000 | ||
| 8 | 1 | 2 | H2O |
