2FL0
Oxidized (All ferric) form of the Azotobacter vinelandii bacterioferritin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.65 |
| Spacegroup name | H 3 |
| Unit cell lengths | 124.480, 124.480, 285.112 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.570 - 2.700 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.095 | 0.289 |
| Number of reflections | 89043 | |
| <I/σ(I)> | 27.8 | 6.8 |
| Completeness [%] | 98.5 | 98.8 |
| Redundancy | 6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 291 | 25% ethanol, 100 mM imidazole, 200 mM Magnesium chloride, Capillary batch method, pH 8.0, temperature 291K |
