2FKW
Structure of LH2 from Rps. acidophila crystallized in lipidic mesophases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9124 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.443, 126.384, 129.705 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.450 |
| R-factor | 0.18651 |
| Rwork | 0.183 |
| R-free | 0.25367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nkz |
| RMSD bond length | 0.030 |
| RMSD bond angle | 1.414 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.536 | 2.540 |
| High resolution limit [Å] | 2.449 | 2.450 |
| Number of reflections | 49795 | |
| <I/σ(I)> | 20.9 | 2.3 |
| Completeness [%] | 99.8 | 98.25 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 200 microlitres of 40 %(w/w) protein, 0.05 %(w/v) LDAO, 20mM Tris/HCl and 60 %(w/w) monoolein mixed with 2 microlitres of precipitant: 20 %(w/v) pentaerythritol propoxylate, 0.1 M potassium formate, 0.1 M Tris/HCl, pH 8.0, Mesophase crystallization, lipidic cubic phase, temperature 293K |
