2FKK
Crystal structure of the C-terminal domain of the bacteriophage T4 gene product 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 69.843, 69.843, 119.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.200 |
| R-factor | 0.12 |
| Rwork | 0.119 |
| R-free | 0.13640 |
| Structure solution method | AB INITIO PHASING |
| Starting model (for MR) | none |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.050 | 0.390 |
| Number of reflections | 105624 | |
| <I/σ(I)> | 20.8 | 2.5 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 5.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 2.3 | 293 | 6% PEG1000, 8% PEG8000, 100 mM glycine-HCl pH 2.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
