2FKH
Crystal Form II of Pre-Reactive Complex of Restriction Endonuclease HinP1I with Cognate DNA and Calcium Ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 109.134, 109.134, 113.269 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.510 - 3.090 |
| R-factor | 0.225 |
| Rwork | 0.220 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ynm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.510 | 3.200 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.071 | |
| Number of reflections | 7757 | |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 16.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 30% PEG4000, 100mM Tris pH 8.5, 0.2M Li2SO4, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4000 | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | Li2SO4 | ||
| 4 | 1 | 1 | CaCl2 | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG4000 | ||
| 7 | 1 | 2 | CaCl2 | ||
| 8 | 1 | 2 | Li2SO4 | ||
| 9 | 1 | 2 | H2O |
