2FKD
Crystal Structure of the C-terminal domain of Bacteriophage 186 repressor
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.606, 167.089, 177.068 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.241 |
Rwork | 0.240 |
R-free | 0.29700 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.870 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 54011 | |
Completeness [%] | 0.9 | 0.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.29 | 298 | 23% PEG 1500, 0.1 M Hepes pH 7.29, 10% sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K |