2FK9
Human protein kinase C, eta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-20 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.007, 49.404, 67.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 1.750 |
| R-factor | 0.17994 |
| Rwork | 0.178 |
| R-free | 0.21458 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gmi |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.629 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.930 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.068 | 0.315 |
| Number of reflections | 15364 | |
| <I/σ(I)> | 31.3 | 3.35 |
| Completeness [%] | 97.5 | 77.5 |
| Redundancy | 6.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 22% PEG3350, 0.2M CaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
