2FK7
Crystal structure of Hma (MmaA4) from Mycobacterium tuberculosis, apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.931000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.715, 56.715, 206.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.19734 |
| Rwork | 0.195 |
| R-free | 0.23226 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kp9 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.736 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 22776 | |
| <I/σ(I)> | 3.8 | 3.7 |
| Completeness [%] | 97.5 | 100 |
| Redundancy | 9.7 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 285 | 4-28% PEG 3350, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K |
