2FK0
Crystal Structure of a H5N1 influenza virus hemagglutinin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 3 |
| Unit cell lengths | 197.943, 197.943, 134.688 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 171.500 - 2.950 |
| R-factor | 0.26898 |
| Rwork | 0.268 |
| R-free | 0.31873 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rd8 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.710 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.480 | 3.020 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 122612 | |
| <I/σ(I)> | 13.6 | 1.7 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 3.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.55 | 290 | 22% PEG 2000, 0.1M HEPES, pH 6.55, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
