2FJY
Crystal Structure of B-form Bombyx mori Pheromone Binding Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Detector technology | CCD |
Collection date | 2002-06-05 |
Wavelength(s) | 0.950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.240, 70.790, 75.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.000 - 2.300 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gm0 |
RMSD bond length | 0.048 |
RMSD bond angle | 2.533 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 12094 |
Completeness [%] | 91.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | NaF, PEG 3350, pH 7.5, hanging drop, temperature 277K, VAPOR DIFFUSION, HANGING DROP |