2FJT
Adenylyl cyclase class iv from Yersinia pestis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Detector technology | IMAGE PLATE |
Collection date | 2004-09-20 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 33.529, 35.548, 71.985 |
Unit cell angles | 88.89, 82.63, 65.47 |
Refinement procedure
Resolution | 9.980 - 1.901 |
R-factor | 0.21608 |
Rwork | 0.212 |
R-free | 0.27293 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | orthorhombic crystal form |
RMSD bond length | 0.017 |
RMSD bond angle | 1.685 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.026 | 0.170 |
Number of reflections | 20986 | |
<I/σ(I)> | 15.7 | 3.9 |
Completeness [%] | 89.3 | 83.9 |
Redundancy | 2.04 | 2.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 297 | 100 mM Ammonium sulfate, 100 mM Sodium acetate, 14% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K |