2FJI

Crystal structure of the C-terminal domain of the exocyst subunit Sec6p

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793, 0.9792, 0.9679
Spacegroup name	P 1 21 1
Unit cell lengths	108.295, 63.301, 151.468
Unit cell angles	90.00, 103.89, 90.00

Refinement procedure

Resolution	15.000 - 2.400
Rwork	0.249
R-free	0.28400
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.073	0.421
Number of reflections	76939
Completeness [%]	98.3	98.2
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1%-4% PEG 6000, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1%-4% PEG 6000, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K