2FJG
Structure of the G6 Fab, a phage derived Fab fragment, in complex with VEGF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 117.880, 117.880, 212.562 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.20077 |
| Rwork | 0.199 |
| R-free | 0.23916 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Search model for VEGF was based on 1FLT search model for the Fab was based on 2FJF. |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.550 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 42763 | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 2.0 M Ammonium sulfate, 5% Isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
