2FJC
Crystal structure of antigen TpF1 from Treponema pallidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 184.921, 184.921, 154.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.370 - 2.500 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ji4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 185.000 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.072 | 0.370 |
| Number of reflections | 111030 | |
| <I/σ(I)> | 8.5 | 1.5 |
| Completeness [%] | 95.7 | 70.8 |
| Redundancy | 5.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 10% PEG 6000, 8% ethylene glycol, 0.1 M Tris buffer, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
