2FJ0
Crystal Structure of Juvenile Hormone Esterase from Manduca sexta, with OTFP covalently attached
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-09 |
| Detector | ADSC QUANTUM |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 96.820, 96.820, 165.395 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 2.700 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.081 | 0.418 |
| Number of reflections | 21455 | |
| <I/σ(I)> | 9.3 | 2.3 |
| Completeness [%] | 96.4 | 97.4 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 14% PEG 3000, 80 mM sodium citrate, 20 mM citric acid, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
